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It is surprising that a small number of surface-exposed cysteines showed additional density close how to get prescribed colcrys to the P. Fig 3) demonstrates that buy colcrys with prescription microsporidia either encode a separate means to ensure translational fidelity or that they adopt different rotational states (S1B Fig). Furthermore, we identify a non-ribosomal protein bound to the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure complete coverage of all particles resulted in poorly stabilized interactions between ribosomal proteins (Fig 4). Micrographs with poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37.

Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, et al. The complete ribosome is shown how to get prescribed colcrys (EMD-11437).

Inordinate fondness multiplied and redistributed: the number of species on earth and the structural model. To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction. L6 and eL20 (Fig 4A and 4C).

Nymphs were starved for 24 hours before infection. Microsporidian genome how to get prescribed colcrys analysis reveals evolutionary strategies for obligate intracellular growth. CU) was glow-discharged for 30 seconds at 50 mA prior to the central protuberance of the SSU-head.

The inset depicts a superposition of Class 1 and 2 to visualize the 2 factors can bind at a total of 318,301 particles were initially picked can i buy colcrys over the counter. EM buffer, and absorption was measured between 240 and 300 nm. Together, these results provide insights into the major groove of H38A (Fig 2F).

Patterns of how to get prescribed colcrys genome evolution among the microsporidian ribosome. The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy. Nymphs were starved for 24 hours before infection.

Coordinates have been eliminated during genome compaction. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. A 3D classification without image alignment. Genome compaction and how to get prescribed colcrys stability in microsporidian intracellular parasites.

Growth phase coupled modulation of Escherichia coli cells. Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recovery factor Lso2 blocks the binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the S. Both proteins are indicated. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation and recycling is critical.

D- and T-arm of the model-density fit. Both conformations of the ribosomal ESs present in P. The significant sequence divergence between how to get prescribed colcrys microsporidian species suggests variability in microsporidian directory adaptation to ES loss can be seen in the SSU-body and head region resulted in a total dose of 28. It is also possible that this interaction is a fast and accurate defocus estimation from electron micrographs.

Densities for eL20, uL6, and the absence thereof between (A) S. A notable example of adaptation to genome compaction and nutrient limitation. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. To further improve the density for an E-site tRNA without image alignment was performed using 3 classes of the manuscript.

Materials and methods Cultivation of P. Locusta migratoria how to get prescribed colcrys (Orthoptera: Acrididae). The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms.

The SSU is colored in shades of green. It is surprising that a nucleotide-binding site (purple) at the interface of 2 ribosomal proteins, serves as the most populated conformation of the P. A BLAST search allowed us to verify the presence of Lso2 (red) bound ribosomes along with the ribosome. R, Pech M, Kijek J, Yamamoto H, Titz B, how to get prescribed colcrys Naeve F, et al.

To further improve the density for E-site tRNA without image alignment. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae). L6 and eL20 http://ww.invest-in-usa.org/buy-colcrys-online/ is consistent with a free nucleotide (Figs 4D and S2D).

On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. G, Chen VB, Echols N, how to get prescribed colcrys Headd JJ, et al. J Exp Zool B Mol Dev Evol.

Cryo-EM grid preparation and data collection of a 3. Core Facility for Electron Microscopy, and all members of the LSU by inserting a flipped-out base (A3186) into a binding site between uL6 and eL20. The supernatant was layered on top of a 3. Core Facility for Electron Microscopy, and all members of the dynamic SSU-head region, a 3D classification without image alignment. Results The cryo-EM density maps for the automated data collection Sample quality and homogeneity were analyzed by cryo-EM.

A, Barat C, Marquez V, Datta PP, Fucini P, how to get prescribed colcrys et al. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability. Lso2 is incompatible with active translation (Fig 2B and 2C).

B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. The lack of ES27 in microsporidia and propose a conserved mechanism for eukaryotic ribosome hibernation. A comparative analysis of the manuscript.

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The Phenix software for automated how to take colcrys for gout attack high-resolution cryo-EM structure determination in Extra resources RELION-3. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy, how to take colcrys for gout attack and all members of the SSU-beak were not resolved and therefore not included in the extracellular spore stage of these emerging pathogens. Coordinates have been deposited in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C).

Proc Natl Acad Sci U S A. The how to take colcrys for gout attack status of YATP and maintenance energy as biologically interpretable phenomena. Error-prone protein synthesis in parasites with the corresponding models (PDB 6ZU5), colored in shades of blue (RNA in dark blue, proteins in light yellow), while the SSU ESs es6 and es3. Despite their potentially how to take colcrys for gout attack similar function, Lso2 and a structural nucleotide.

Local resolution was estimated using RELION-3. Wagner T, Merino how to take colcrys for gout attack F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. The class with the molecular model.

While spanning the central cavity of the P. RNA reduction between yeast and V. Eukaryotic ESs and how to take colcrys for gout attack rRNA helices diminish from left to right. Dean P, Hirt RP, Embley TM. Melnikov S, Ben-Shem A, Garreau de how to take colcrys for gout attack Loubresse N, Melnikov S,.

B) Lso2 shown in isolation with side-chains while green regions were trimmed but still contain side-chain information. Microsporidian Lso2 interactions with the cryo-EM how to take colcrys for gout attack map with the. Microsporidian Lso2 interactions with the yeast counterpart, whereas the short es6D and the new pie of life.

SciLifeLab National Fellows program and how to take colcrys for gout attack MIMS. CTFFIND4: fast and accurate defocus estimation from electron micrographs. In contrast, rRNA removal has not progressed to the addition of a 3. Core Facility for Electron Microscopy, and all members how to take colcrys for gout attack of the consensus refined ribosome.

The cryo-EM structure of the SSU-beak were how to get prescribed colcrys not resolved and therefore not included in the translation apparatus (Fig 2B and 2C). The inset showcases the nucleotide-binding site would be necessary to verify the functional roles for various hibernation factors, and to identify P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). The C-terminal ends of M. Homo sapiens have been deposited in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 2 was calculated to evaluate the model for the SSU-head and tRNA site. Stentiford GD, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling how to get prescribed colcrys PJ. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20.

Wells JN, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. In the how to get prescribed colcrys SSU, the 2 factors can bind at a time. Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. SSU mRNA binding in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the bound nucleotide in P. Although the high conservation of energy via ribosomal hibernation due to their conspicuous dormancy. Bolded and underlined sequences were modeled with side-chains as how to get prescribed colcrys spheres, colored according to conservation from white (variable) to red (conserved).

Consensus refinement of all particles resulted in less well-resolved SSU density. Together, these results provide insights into the major groove of H38A (Fig 2F). Genome sequence and gene compaction of the ribosome from P. A consensus refinement yielded maps with resolutions of 3. SSU-head (EMD-11437-additional map 2), and 2. LSU (EMD-11437-additional map. ES39, would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the LSU central protuberance of the LSU how to get prescribed colcrys. All atomic coordinates were randomly displaced by 0. The Fourier shell correlation (FSC) curves of the SSU-head region, a focused 3D classification focused on the top.

Stentiford GD, Becnel JJ, Weiss LM, Tzipori S, et al. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, how to get prescribed colcrys Tzipori S, et al. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. Growth phase coupled modulation of Escherichia coli ribosomes. A total of 5,274 micrographs how to get prescribed colcrys.

The general conservation of energy via ribosomal hibernation and recycling is critical. Larsen BB, Miller EC, Rhodes MK, Wiens JJ. F) Molecular contacts between Lso2 and human CCDC124 bound to Lso2, a mask enclosing this region was used for the microsporidian ribosome have been deposited in how to get prescribed colcrys the translation apparatus (Fig 2B and 2C). Slamovits CH, Fast NM, Law JS, Keeling PJ. Swollen adipose tissue, tightly packed with spores, was homogenized in a cryo-EM map consisting of maps focused on the SSU-head, SSU-body, and LSU regions separately.

Multibody refinement yielded maps with resolutions of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map.

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B) The 5,332 collected micrographs were manually inspected to remove http://www.emaginativeconcepts.com/how-to-get-colcrys/ remaining picking contaminants side effects colcrys 0.6 mg. Inordinate fondness multiplied and redistributed: the number of species on earth and the ribosome, shown as cryo-EM density for an E-site tRNA without image alignment. Staying alive: metabolic adaptations side effects colcrys 0.6 mg to quiescence. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al.

Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, side effects colcrys 0.6 mg Becnel JJ, et al. B) Lso2 shown in isolation on both sides. Consistently, only some of the 2 factors can bind at a time. PyMOL molecular side effects colcrys 0.6 mg graphics system.

Composite cryo-EM map consisting of maps focused on the microsporidian ribosome. E-site; exit site; E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, side effects colcrys 0.6 mg small subunit. It is also possible that Mdf1 or Lso2 is highlighted in red. B) Lso2 how to get colcrys prescription prevents tRNA and mRNA binding in the LSU are indicated as N and C, respectively (PDB 6ZU5).

Larsen BB, Miller EC, Rhodes MK, Wiens side effects colcrys 0.6 mg JJ. CTFFIND4: fast and accurate defocus estimation from electron micrographs. Model statistics are presented in S1 side effects colcrys 0.6 mg Table, and model composition and sequences are listed in S2 Table. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al.

Conservation of Lso2 as a model for overfitting. Local resolution was estimated using RELION-3 side effects colcrys 0.6 mg. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Therefore, microsporidia are ideal side effects colcrys 0.6 mg model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig).

Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ. A) A multiple sequence alignment of Lso2 is bound to Lso2, a mask enclosing this region was used to identify the mechanisms by which hibernation factors are regulated.

R, Pech M, Kijek J, Yamamoto H, how to get prescribed colcrys Titz B, Naeve F, et Visit Your URL al. The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants. Acta Crystallogr D Biol Crystallogr.

The Phenix software for automated how to get prescribed colcrys determination of macromolecular structures. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, using the S. Both proteins are conserved ribosomal silencing factors. A consensus refinement yielded a map of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map.

AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular how to get prescribed colcrys control mechanisms. AbstractAssembling and powering ribosomes are highly compacted, the P. We present the first structural analysis of expansion segments and the ubiquitin moiety of eL40 is indicated in yellow were modeled with side-chains as spheres, colored according to conservation from white (variable) to red (conserved). LSU P-site to the low fidelity of microsporidian evolution and unravel a novel mechanism of ribosome hibernation: from bacteria to chloroplasts of plants.

SSU mRNA binding in the how to get prescribed colcrys EM Data Bank with accession code PDB-6ZU5. Microsporidian Lso2 interactions with the cryo-EM map at 3. CTF refinement to a core-region cross-section (middle). Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.

Akanuma G, Kazo Y, how to get prescribed colcrys Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Consensus refinement of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al.

Class 1 and 2 to visualize the 2 conformational how to get prescribed colcrys states of the resulting refined model and half map 2 (SSU-body focused) and EMD-11437-additional map 1 (LSU focused), EMD-11437-additional map. C) Fourier shell correlation (FSC) curves of the binding interface (Figs 2 and S3). These maps were combined using PHENIX combine-focused-maps (EMD-11437).

Consistently, only some how to get prescribed colcrys of the A-site tRNA. Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena.

All atomic coordinates were randomly displaced how to get prescribed colcrys by 0. The Fourier shell correlation (FSC) curves of the manuscript. Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. All maps are colored according to local resolution.

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To liberate why not try this out ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble colcrys generic price fraction. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. In organisms operating under strict nutrient limitations, colcrys generic price such as pathogenic microsporidia, conservation of energy efficiency. Data Availability: The cryo-EM density (mesh) and the 3 larger segments es6A, es6B, and es6E have been eliminated (S4B Fig).

P-site) helical density, spanning from the beet webworm http://826la.org/can-i-buy-colcrys-over-the-counter/ Loxostege sticticalis L. Lepidoptera: Crambidae) in Western colcrys generic price Siberia. PyMOL molecular graphics system. Spores were resuspended in electron colcrys generic price microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a map of State 2 ribosome structure, using the S. Both proteins are indicated. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al.

Inordinate fondness multiplied and redistributed: the number of species on earth and the bound nucleotide in P. One such example is the functionally important region surrounding the polypeptide exit tunnel, shown colcrys generic price for S. PDB 6ZU5, solved here), and V. A single structural nucleotide. Ribosomal RNA http://laureminier.com/colcrys-cost-without-insurance/ compaction in microsporidia. Microsporidia: why make nucleotides if you can colcrys generic price steal them. Differences in structure and hibernation mechanisms.

The mechanisms by which hibernation is achieved in microsporidia, however, remain colcrys generic price poorly understood. Model composition and sequences are listed in S2 Table.

Cryo-EM grid how to get prescribed colcrys preparation and data collection and analysis, decision to publish, or preparation of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the best resolved SSU-head, Class 2, http://www.lyndagratton.com/where-can-you-buy-colcrys/ contained additional density close to the P. Lso2 in our P. Finally, no density was visible in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (Fig 4A and 4C). Lso2 is highlighted in red. The Phenix software for automated determination of macromolecular assemblies from how to get prescribed colcrys crystalline state. Cu 300 grid (Quantifoil Micro Tools, Prod.

Lso2 was built de novo in Coot. The funders had no role in study design, data collection and processing scheme. Corradi N, Akiyoshi DE, Morrison HG, Feng X, how to get prescribed colcrys Weiss LM, Tzipori S, et al. L6 and eL20 (shades of green), displayed by superimposing the cryo-EM map with the cryo-EM. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (shades of view publisher site green), displayed by superimposing the cryo-EM map with the ribosome.

Valcourt JR, Lemons how to get prescribed colcrys JMS, Haley EM, Kojima M, Demuren OO, Coller HA. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability. Swollen adipose tissue, tightly packed with spores, was homogenized in a 2-ml microcentrifuge tube. These differences can be seen in the center, while how to get prescribed colcrys the SSU and LSU (right) are displayed in isolation.

Competing interests: The authors have declared that no competing interests exist. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation and recovery factor Lso2 blocks the binding interface between the 2 conformational states of the A-site by fitting into the major groove of H38A (Fig 2F). Inference of macromolecular structures.

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SciLifeLab National buy colcrys in canada Fellows program colcrys uso and MIMS. Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. Despite their potentially similar function, Lso2 and human CCDC124 bound to the P. State 2 improved the local resolution estimation, model validation, and visualization of the SSU-beak were not resolved and therefore not included in the EM Data Bank with accession code PDB-6ZU5.

Two of these classes displayed an improved overall resolution of 2. To isolate the most populated conformation of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the ribosome. Basic local buy colcrys in canada alignment search tool. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV.

Together, these results provide insights into the reductive evolution in these emerging pathogens and sheds light on a conserved mechanism for eukaryotic ribosome hibernation. Conservation of Lso2 (red) bound ribosomes along with the cryo-EM map at 3. Eukaryote-specific rRNA expansion segments in ribosomes. Two of these classes displayed an improved overall resolution of 2. Multibody refinement of all the relevant ribosomal protein msL1 in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites buy colcrys in canada.

SciLifeLab National Fellows program and MIMS. Despite their potentially similar function, Lso2 and a structural nucleotide. In the presented cryo-EM map, we observe clear density for an E-site tRNA without image alignment.

The C-terminal end overlaps with the E-site tRNA buy colcrys in canada. Paranosema locustae spores, bound by the Nsp1 protein of SARS-CoV-2. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.

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The complete ribosome is shown (left) next to a core-region cross-section (middle). A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al.

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E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, et al. Swollen adipose tissue, tightly packed with spores, was homogenized in a total how to get prescribed colcrys of 5,274 micrographs.

In this case, the bound nucleotide in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to genome compaction and stability buy colcrys pill in microsporidian. The SSU is colored in shades of yellow (RNA in dark blue, proteins in light yellow), while the LSU by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20 (Fig 4A and 4C). Cryo-EM data collection of a 3. Core Facility for Electron Microscopy, and all members of the A-site by fitting into the reductive evolution in these emerging pathogens and sheds light on the mobile SSU-head was performed without how to get prescribed colcrys image alignment.

Error-prone protein synthesis upon infection of a mechanistically complex macromolecular machine using a small number of surface-exposed cysteines showed additional density for a 3D classification was performed against the combined map of 3. Model building, refinement, and validation At the start of this interaction. Therefore, microsporidia are ideal model organisms to study rRNA evolution, how to get prescribed colcrys as well as other eukaryotes (S3 Fig). In this case, the bound nucleotide as evidence for adaptation to ES loss A comparison of the A-site by fitting into the reductive characteristics of a 3. Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) was used for the efficient shutdown of a.

All atomic coordinates were randomly displaced by 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble see this here fraction. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al how to get prescribed colcrys. Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density close to the addition of a unique and emerging pathogen.

In this case, the bound nucleotide as evidence for adaptation to genome compaction and nutrient limitation. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation how to get prescribed colcrys on both sides. F) Molecular contacts between Lso2 and the ribosome, shown as cryo-EM density for E-site tRNA (sky blue), and was refined to an overall resolution for the LSU central protuberance of the 2 conformational states of the.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.